What We Do?
PLANT PATHOGEN SCREENING
Breeding new crop varieties. Tissue culture was used to solve the problem of seed embryo failure in crossbreeding and to obtain hybrid progeny, which made distant hybridization successful. Haploid plants were obtained by anther culture and in vitro culture of unpollinated ovaries, thus opening the way to haploid breeding. Obtaining disease-free plants. In the case of nutrient-propagated crops, viruses can be transmitted to the next generation through the nutrient organs used for reproduction, causing crop degradation and yield reduction as the number of crop generations accumulates and even leading to the extinction of some varieties. Based on the uneven distribution of viruses in plants, virus-free plants can be obtained using tissue culture with growing points combined with virus identification. Preservation and transportation of germplasm. With the tissue culture method, there is no need to preserve germplasm resources in the same way as seeds, and plant organs, tissues, and even cells can be preserved for a long period under low or ultra-low temperature conditions. Tissue culture can be used for rapid propagation when needed in the future. Plant tissue culture technology is a very effective technical tool in breeding and detoxification of good seeds, production of secondary metabolites, and preservation of seed resources. However, the strict requirements for the aseptic environment in plant tissue culture technology have resulted in high investment in tissue culture production, complicated operation procedures, and serious contamination, which have restricted the application and popularity of this technology.
PROTOPLAST ISOLATION AND CULTURE FOR CROP IMPROVEMENT
Cannabis breeding can be a complex and intricate process that requires a deep understanding of genetics and plant biology. While basic breeding techniques may be sufficient for some, others may choose to explore more advanced methods to achieve their desired results.
One such technique is backcrossing, which involves crossing a hybrid offspring with one of its parent strains. This is done to reinforce specific traits from the parent strain in the hybrid offspring while maintaining the desired traits from the previous generation. Line breeding is another advanced method that involves breeding plants with a shared ancestor to maintain genetic stability and reinforce desirable traits. This technique is frequently used to create stabilized strains with consistent characteristics. Selection for specific traits is also an advanced breeding method that involves identifying and selecting plants that exhibit desired characteristics such as high THC content or unique terpene profiles. This technique involves a meticulous process of observation, documentation, and selection over many generations. While advanced breeding methods can be challenging, they offer breeders the opportunity to create truly unique and customized strains with specific characteristics. They require patience, dedication, and a willingness to experiment and learn.
Plant tissue culture techniques can be used in plant breeding efforts to meet growing global food demands. Protoplast isolation and culture allows plant breeders to overcome the challenges of traditional breeding and to facilitate gene transfer that permits testing for desirable characteristics. Protoplasts are cells which lack cell walls and can be used in plant tissue culture systems to regenerate whole plants from a single cell. Crop improvement efforts to increase quality, quantity and efficiency for agricultural crop production can achieve desired hybrids using protoplast fusion, isolation and culture despite incompatibility issues in traditional plant breeding